Competition for water sources in urban areas of Florida has increased due to increased population and human activities. High water users have been identified as a specific group on which Extension should focus water conservation education due to their low awareness of water issues and active landscape water use. In order to ensure the effectiveness of Extension programs targeting high water users statewide, this study sought to explore regional differences in water conservation behavior engagement within Florida high water users. An online survey was conducted to capture responses of high water users (N = 932) in three distinct regions for this comparative study. Respondents were asked to indicate their current engagement in water use behavior, application of water conservation strategies, and likelihood of engaging in water conservation and related societal behaviors. Regional differences were found in all four examined constructs. The findings imply Extension educators should tailor educational programs to regional audiences’ behavior patterns instead of designing statewide programs to ensure program effectiveness.